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KMID : 0381919960260040401
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Korean Journal of Microscopy
1996 Volume.26 No. 4 p.401 ~ p.410
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Fixation of Cellular Ultrastructure by the Microwave Irradiation
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Shin Kil-Sang
Kim Wan-Jong
Jeon Jin-Seok
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Abstract
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The microwave fixator has recently been introduced in morphological research. The present study was carried out to investigate the ultrastructural effects of microwave fixation of rat brain. kidney, liver and skeletal muscle tissues. The results are as follows: In the case of microwave fixed cerebrum. the cytoplasmic processes of neurons and the various membranous organelles such as nuclear envelope, mitochondria, rough endoplasmic reticulum and Golgi apparatus were well preserved, The myelin sheath wrapping neuronal axon was prominent. Microwave fixed hepatocytes showed the microvilli on the free surface of bile canaliculus, the evident nucleolar components, and typical organelles. In nephron, ultrastructures of glomerulus and Bowman’s capsule were preserved, and also tubular wall were structurally observed. Among the skeletal muscle cells, plentiful collagen fibers were appeared, myofibrils and mitochondria were typically observed. In conclusion, the microwave fixation procedures result in an good preservation of the tissues and would be time- and reagent-saving.
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KEYWORD
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Microwave, Ultrastructure, Tissues, Preservation
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